Benevolent Approach of In Vitro And Ex Vivo Study in Ayurvedic Ocular Therapeutics

Abstract

Animal Experimentation like in vitro and in vivo study plays an important role in research and understanding the ocular delivery system. Any new drug development is carried out on cell culture. Tissue engineering has developed biologically equivalent replacements which are used for research. Topical therapies in the form of kriyakalpa are widely used in netra roga chikitsa. There are multiple studies available regarding the results of kriyakalpa. It is need of hour to prove its mode of action, ocular drug delivery, safety, efficacy and toxicity of ayurvedic ocular medicines on modern parameters. If proven; ayurvedic ocular therapeutics will be globally accepted. Many ocular therapeutics give temporary symptomatic relief, in such conditions alternative ayurvedic treatment modality can be developed using cell culture study. In vitro and ex vivo models of the conjunctiva, cornea, vitreous, retina, etc can play an important role in research and development of ayurvedic ophthalmic drug. This review summarizes various available cell models, their uses and limitations. This can be helpful for further research in ayurvedic ocular drug delivery system.