Impact of Carbohydrate and Amino Acid Enriched Diet on The Heat Tolerance of Drosophila Ananassae and Drosophila Bipectinata

Abstract

Elevated temperatures promote the accumulation of damages, which can be countered by improving diet. So, it is critical to comprehend how environmental temperature influences the survival of an organism. Providing a diet with additional nutrient regimens accelerates the physiological and metabolic processes in organisms, which results in better survival. Present study aimed to understand the impact of carbohydrate and amino acid-enriched diet on the heat tolerance of Drosophila ananassae and D. bipectinata. Adult flies were transferred to the media composition enriched with carbohydrate and amino acid in three replicates and maintained at 22^oC. 20 flies of a particular stage in three replicates were exposed to 24^oC, 28^oC, and 32^oC for 5 days. The impact of thermal stress on their survival was noted for 5 following days. The results of this study show different stages of Drosophila ananassae and D. bipectinata can handle heat stress in a better manner when fed with a carbohydrate and amino acid-rich diet. D. bipectinata survival rates were higher than D. ananassae when compared at each temperature at different diet regimens. This study provided convincing evidence of the positive influence of the tryptophan (100mg) diet compared to the sucrose diet on the survival, quality of life, and stress tolerance of both species.