Localization Of Transforming Growth Factor-β 1 In The Testis Of Freshwater Catfish, Clarias Batrachus, And Its Relation With Testicular Activity
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Abstract
TGF-β1, an isoform of transforming growth factor β, is involved in regulation of variety of physiological functions. In mammals, TGF-β1 is known to regulate reproductive activity but its role in reproduction of lower vertebrate, particularly fishes, is not yet clear. Therefore, study was planned to detect its presence in the testis of catfish, Clarias batrachus during different phases of its reproductive cycle. Expression of TGF-β1 was visualized through the immunohistochemistry along with the measurement of testicular testosterone. The immunoreactivity was present in Sertoli cell and germinal epithelium. As the testicular activity progressed the expression of TGF-β1 decreased gradually in testis. However, the level of testicular testosterone increased with the progression in spermatogenesis. This finding suggests that TGF-β1 is present in fish testis and its expression varies with changing reproductive status; it expresses more during the early reproductive phase. The correlation studies showed a negative correlation between TGF-β1 expression and testicular testosterone production.
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